增精颗粒对大鼠附睾精子质量的影响

Effects of ZJG on the Quality of Sperm in Male Rat Epididymis

目的 :观察中药增精颗粒 (ZJG)对不育大鼠附睾精子质量的影响 ,探讨ZJG改善附睾精子质量的机制。 方法 :将 4 0只雷公藤多甙 (GTW )实验性SD雄性大鼠不育模型随机分成 4组 ,每组 10只 ,包括不育组 [GTW 2mg/(ml·10 0g) ]和ZJG高、中、低剂量组 [高 :ZJG0 .6 7g/(ml·10 0g) +GTW 2mg/(ml·10 0 g) ,中 :ZJG0 .33g/(ml·10 0g) +GTW 2mg/(ml·10 0 g) ,低 :ZJG0 .17g/(ml·10 0 g) +GTW 2mg/(ml·10 0g) ],另设正常组 (对照 ) 10只(等量的 1%羧甲基纤维素钠溶液 )。整个实验周期 6周 ,由 3周造模期和 3周用药期组成。ZJG各组按指定的剂量用药 3周 (实验第 4 3d)观察大鼠附睾精子质量、附睾腺管壁厚度及主要性腺器官系数的变化。 结果 :ZJG高、中、低剂量组附睾精子密度分别为 (5 9.6± 3.72 )、(6 3.3± 5 .70 )、(6 9.7± 6 .91)× 10 6/ml,精子活率分别为 (6 5 .4±6 .33) %、(6 9.3± 10 .96 ) %和 (72 .6± 9.6 1) % ,畸形率分别为 (5 2 .3± 7.4 7) %、(4 6 .2± 7.73) %和 (33.2± 7.97) % ,与不育组 [精子密度 (13.1± 6 .81)× 10 6/ml,精子活率 (7.6± 5 .87) % ,畸形率 (77.2± 8.75 ) % ]相比 ,ZJG各组差异均有显著性 (P <0 .0 5 ) ,与正常组 [精子密度 (75 .6± 10 .82 )× 10 6/ml,精子?

Objective: To observe the effects of Chinese herbal composition Zengjing Granule (ZJG) on the quality of sperm in the epididymis of infertile rats, and to study its therapeutic mechanisms of improving sperm quality. Methods: A total of 40 GTW infertile rats were divided into 4 groups of 10 rats each, including an infertility group[GTW 2 mg/(ml·100 g)], a high-dosage group [ ZJG 0.67 g/(ml·100 g)+GTW 2 mg/(ml·100 g)], a medium-dosage group [ZJG 0.33 g/(ml·100 g)+GTW 2 mg/(ml·100 g)],a low-dosage group [ ZJG 0.17 g/(ml·100 g)+GTW 2 mg/(ml·100 g)], and a normal control group(1%CMC). This study consisted of a 3-week modeling period and a 3-week ZJG period. The changes of sperm quality, the thickness of the epididymal gland canal wall and sexual organ coefficient were detected after 3-week ZJG period. Results: Of the 3 ZJG groups, the sperm density was ( 59.6± 3.72), ( 63.3± 5.70) and ( 69.7± 6.91) ×10 6/ml, the sperm motility rates were ( 65.4± 6.33)%, ( 69.3± 10.96)% and ( 72.6± 9.61)%, the sperm deformity rates were ( 52.3± 7.47)%, ( 46.2± 7.73)% and ( 33.2± 7.97)% repectively. The ZJG groups showed significant difference from the infertility group (P< 0.05), whose sperm density, motility and deformity were ( 13.1± 6.81)×10 6/ml, ( 7.6± 5.87)%, and ( 77.2± 8.75)% respectively. But there was no significant difference between ZJG groups and the normal control group(P> 0.05), whose sperm density, motility and deformity were ( 75.6± 10.82)×10 6/ml, ( 83.00± 8.02)%, and ( 8.80± 3.49)% respectively. The thickness of the epididymal gland canal wall was ( 37.07± 3.38), ( 37.16± 6.69) and ( 43.42± 10.23) nm in the three ZJG groups respectively, different from the infertility group[ ( 28.65± 6.96) nm] (P< 0.05) significantly, but not from the normal control group [( 45.79± 11.13) nm] (P> 0.05). The ZJG groups showed an increase in the organ coefficient of the epididymal gland canal wall. And these was obvious statistical difference compared with the infertility group (P< 0.05), but no statistical significance compared with the normal control group (P> 0.05). Conclusions: ZJG can obviously improve the sperm quality of infertile rats. Its therapeutic mechanisms can be summed up as follows: restoring the thickness of epididymal gland wall, increasing the organ coefficient of testes and epididymis, and hence improving the spermatozoa maturing function of epididymis of infertile rats.