摘要
目的 :研究和探讨突变型 p5 3、C- myc和血管内皮生长因子 (VEGF)基因蛋白在胶质瘤中的表达和在胶质瘤发生过程中的作用及临床病理意义。 方法:6 5例胶质瘤患者分为低级别组 ( ~ 级 ) 2 8例 ,高级别组 ( ~ 级 ) 37例 ,采用免疫组织化学法检测两组胶质瘤 p5 3、C- myc、VEGF 3种基因蛋白的表达 ,并进行统计学分析。结果 :突变型 p5 3、C- m yc和 VEGF在胶质瘤中的阳性表达率分别为 6 3% (39/6 2 )、6 0 % (35 /5 8)、72 % (4 4 /6 1)。两组VEGF、C- m yc的阳性表达率差异有统计学意义 (P <0 .0 1) ,突变型 p5 3的表达在两组间差异无统计学意义 (P >0 .0 5 ) ,VEGF的产生与 p5 3突变和 C- myc过度表达之间无相关性 (P >0 .0 5 )。结论:p5 3突变和 C- m yc过度表达在胶质瘤发生过程中起重要的作用 ,肿瘤组织产生和分泌 VEGF在胶质瘤快速生长、恶性增殖过程中起关键作用 ,通过阻断 VEGF或其受体抑制成血管过程 ,有望对临床治疗胶质瘤提供一种新途径。
Objective: To explore the role and clinicopathological significance of mutant p53 gene,C-myc gene and VEGF expression in the development of human intracranial glioma. Methods: Expression of p53,C-myc protein and VEGF in tumor tissues of 62 patients with intracranial gliomas was detected by immunohistochemistry technology(LSAB). Results: The frequency of positive immunoreactivity of p53,C-myc and VEGF were 63%(39/62),60%(35/58),72%(44/61) respectively. Expression of VEGF and C-myc revealed a significant difference between benign (Ⅰ-Ⅱ) and malignant(Ⅲ-Ⅳ) gliomas (P<0.01). No correlationship between p53,C-myc protein expression with VEGF was found respectively (P> 0.05). Conclusions: p53 genetic mutation and overexpression of C-myc may play an important role in tumorigenesis of human gliomas. Production and secreting of VEGF in tumor tissues may be a critical factor for the rapid growing and malignant progressing in human intracrainal gliomas. Blocking the neovascularization by anti-angiogenesis agents may offer a new trial for glioma therapy.
出处
《新疆医科大学学报》
CAS
2004年第1期43-46,共4页
Journal of Xinjiang Medical University