重离子辐照对红酵母的诱变作用

A function of mutagensis on rhodotorula RY strain irradiated by heavy ion

应用重离子加速器的 50MeV/u12C6+重离子对胡萝卜素生产菌-红酵母(Rhodotorula RYStrain )进行辐照处理,经酵母发酵实验,发现 50MeV/u12C6+重离子对胡萝卜素生产菌-红酵母具有诱变作用。初步筛选到了胡萝卜素产量有变化的辐照变异菌株,并对这些辐照变异菌株进行了 RFLP(限制性片段长度多态性)和 RAPD(随机扩增 DNA 多态性)分析,这些工作为工业上利用重离子对胡萝卜素生产菌进行诱变育种展现了新的前景。

In this paper, red yeast (Rhodotorula RY Strain) that produces carotene is irradiated by 50MeV/u12C6+ heavy ion from Heavy Ion Accelerator in IMP. Fermentation tests show that 50MeV/u12C6+ heavy ion has a mutagenesis effect on the red yeast. Some strains of red yeast with changed production of carotene were found by screening. Meanwhile, by RFLP and RAPD analysis, we have a further evidence that heavy ion can cause mutagenesis in Rhodotorula RY Strain. This presents a new prospect for the mutagenesis breeding by heavy ion in industry.