MEK的抑制剂U0126可部分阻断bFGF对辐射诱导的血管内皮细胞凋亡的抑制作用

MEK inhibitor U0126 may partly block the inhibitory effect of bFGF on radiation-induced apoptosis in endothelial cells

为研究 bFGF(碱性成纤维细胞生长因子)对辐射诱导的血管内皮细胞凋亡的作用及其信号转导途径,以 Coγ 射线照射法建立人脐静脉内皮细胞株 ECV304 细胞凋亡模型,以流式细胞术(Annexin-V-FITC+PI 标 60记)检测细胞凋亡率。观察不同浓度的 bFGF 和 MEK 的特异性抑制剂 U0126 对细胞凋亡的影响。结果显示,bFGF 对辐射诱导的 ECV304 细胞凋亡有明显的抑制作用,而 U0126 可阻断 bFGF 的抗凋亡作用。提示 bFGF可能通过 MAPK 信号转导途径抑制辐射诱导的血管内皮细胞凋亡。

In order to investigate the effect of bFGF on radiation-induced apoptosis in vascular endothelial cells and its signal transduction pathways, ECV304 cells were irradiated with Co γ rays for establishing cell apoptosis 60 model. Flow cytometry with Annexin-V-FITC-PI labeling was used to determine cell apoptosis. Different amounts of bFGF and MEK inhibitor U0126 were used to study their effects on radiation-induced endothelial cell apoptosis. It was found that bFGF could inhibit radiation-induced apoptosis in ECV304 cells, but U0126 could block the anti-apoptosis effect of bFGF, suggesting that bFGF may inhibit radiation-induced apoptosis in vascular endothelial cells through of MAPK signaling.