海南粗榧愈伤组织的诱导和培养

Callus Induction and Culture of Cephalotaxus mannii

海南粗榧的嫩茎和嫩叶以0.1%升汞消毒12min的效果最好。外植体在MS+0.1mg.L-16-BA+1mg.L-1NAA+2mg.L-12,4-D+3.0%蔗糖+0.6%卡拉胶(pH5.8)培养基中能顺利诱导出愈伤组织。液体悬浮和暗培养均有利于愈伤组织的增殖。愈伤组织增殖的最佳培养基为MS+0.1mg.L-1KT+3mg.L-1NAA+3.0%蔗糖(pH5.8)。

The results showed that: (1)Young stem and young leaf of Cephalotaxus mannii were sterilized bestin 0.1% mercuric chloride for 12 min; (2)MS medium+0.1 mg.L-1 6-BA+1 mg.L-1 NAA+2 mg.L-1 2,4-D+3.0%sucrose+0.6% carrageenan (pH 5.8) could induce calli from explants; (3)Liquid suspension and dark cultureswere advantageous to proliferation of callus; (4)MS medium +0.1 mg.L-1 KT+3 mg.L-1 NAA+3.0% sucrose(pH 5.8) was optimum for proliferation of callus.