迷你玫瑰组织培养中细菌污染的防止

Control of Bacterial Contaminants in Tissue Cultures of Rosa hybrida cv.mini

迷你玫瑰组织快繁体系连续继代过程中，插入培养基的试管苗切口处有云雾状污染菌生长，其中2种为革兰氏阴性杆菌，1种为革兰氏阳性杆菌。培养基中加入氨苄青霉素(50～150mg·L^-1)抑制污染菌生长，不影响试管苗不定芽分化率，50mg·L^-1的氨苄青霉素明显促进染菌苗的生根。加入硫酸丁胺卡那霉素(25～100mg·L^-1)有杀灭污染菌的作用，但对试管苗则有不同程度的毒害(叶片、叶柄和茎段出现黄化现象)，对不定芽分化和生根也有明显的抑制。

Bacterial contamination is observed as faint clouds in the medium after repeated subculture periodof mini-rose micropropagation system. The contaminants are purified and identified as two different kinds ofGram-negative and one kind of Gram-positive rod bacteria. The two antibiotics, ampicillin and kanamycinsulphate which concentrations are respectively 50~150 mg.L-1 and 25~100 mg.L- 1 are used in the culture media.The result shows that ampicillin has bacteriostatic effect on contaminants and that the effect on adventitious-bud differentiation of contaminated plants after the treatments of different ampicillins concentrations has alsono evident difference as that of normal plants and the treatment concentration (50 mg.L-1) greatly advances theroot formation rate of the contaminated plantlets. The treatments of different concentrations of kanamycinsulphate well kill the contaminants and have phytotoxic influence on the contaminated plantlets, which leaves,shoots and stems turn yellow. They have also greatly reduced adventitious-bud differentiation and their rootformation rate.