哮喘发作患儿CD_(30)分子表达及与Th2源细胞因子和血浆免疫球蛋白E水平的相关性研究

The relationship of expression of CD_(30) on CD4^+ cell surface and level of Thelper cell 2 derived cytokine and plasma total immunoglobulin E in asthmatic children

目的 探讨CD3 0 在哮喘发病中的作用。方法 随机选择哮喘急性发作期患儿 2 7例 ,急性上呼吸道感染 (上感 )患儿 16例 ,对照组 19例。采用直接免疫荧光流式细胞术检测外周血单核细胞 (PBMC)中CD4+ 细胞表达CD3 0 百分率 ,采用ELISA法检测培养上清IL 4、IL 13及血浆IgE水平。 结果 1.哮喘患儿PBMC中CD4+细胞表达CD3 0 百分率较对照组和上感组明显增高 ,差异有显著性 (P均 <0 .0 5) ;2 .哮喘患儿PBMC培养上清IL 4、IL 13和血浆总IgE水平均较对照组和上感组增高 ,上感组血浆IgE水平亦较对照组增高 ,差异均有显著性 ;3 .哮喘组CD4+ 细胞表达CD3 0 百分率与培养上清IL 4、IL 13和血浆IgE水平呈显著正相关。 结论 分泌IL 4和IL 13的Th2类细胞活化、增殖的克隆可能主要是由CD3 0 阳性细胞克隆组成 ,Th2细胞表面CD3 0 与CD3 0 L结合后导致Th2细胞分化成熟及释放Th2源细胞因子 ,IL 4和IL 13增加可诱导B细胞分泌较多的IgE。说明CD3 0

Objective To explore the role of CD 30 on CD4 + T lymphocyte and T lymphocyte subset activation in asthma pathogenesis.Methods Twenty seven asthma active attack patients,16 acute upper respiratory infection patients and 19 control children were randomly selected. Direct immunofluorescence flow cytometry was used to detect the CD 30 positive percentage on CD4 +cell; ELISA was used to detect the levels of interleukin(IL) 4 and IL 13 in culture supernatants of peripheral blood mononuclear cells(PBMC) and plasma total immunoglobulin E(IgE) level.Results Compared with control group and acute upper respiratory infection group, in asthmatic children the CD4 +CD 30 + percentage on CD4 + cell was elevated significantly; 2.The levels of IL 4 and IL 13 in supernatants of cultured lymphocyte were increased significantly, the plasma total IgE level was increased significantly;3.There was a significant positive correlation of CD4 +CD 30 + cell percentage with the levels of IL 4 and IL 13 in culture supernatants and plasma total IgE.Conclusions The cell that could produce Th2 derived cytokine may consist of CD 30 +cell;When CD 30 L combined with CD 30 on CD4 + cells ,it might result in Th2 cell proliferation ,develop and release Th2 derived cytokine; IL 4 and IL 13 could induce B cell production and secrete more IgE. It is suggested that CD 30 and imbalance of Th2 subset may play an important role in asthma pathogenesis.