雄激素致不孕大鼠胰腺、下丘脑及卵巢雄激素受体mRNA表达

Expression of Androgen Receptor mRNA in Pancreas, Hypothalamus and Ovary of Androgen Sterilized Rat

目的:研究雄激素致不孕大鼠(ASR)胰腺、下丘脑及卵巢组织中雄激素受体(AR)mRNA的含量变化。方法:9日龄SD雌性大鼠皮下注射丙酸睾丸酮制备ASR模型,于106日龄左右(动情前期)处死,取血放免法测定△4-雄烯二酮(△4-A)、总睾酮(TT)、游离睾酮(FT)、胰岛素(Ins)和C-肽(C-P),提取胰腺、下丘脑及卵巢总RNA,以单碱基突变模板为内对照的RT-PCR方法对ARmRNA进行定量分析。结果:ASR模型血△4-A、TT、FT、Ins、C-P均显著高于对照组(P<0.05,P<0.01),胰腺、下丘脑及卵巢的AR mRNA表达水平明显升高,与对照组相比差异有统计学意义(P<0.05,P<0.01)。结论:ASR模型血雄激素水平升高,上调胰腺、下丘脑及卵巢AR mRNA的表达,引起该模型的高胰岛素血症和无排卵。

Objective: To investigate the expression of androgen receptor (AR) mRNA in pancreas, hypothala mus and ovary of androgen sterilized rats (ASR). Methods:ASR model was established by subcutaneous injection of testosterone propionate into SD female rats at the age of 9 days. Around the age of 106 days (proestrogenic phase), all rats were sacrificed, serum △4-andronestedione (△4-A), total testosterone (TT), free testosterone (FT), insulin (Ins) and C-peptide (C-P) were measured by radioimmunoassay (RIA). Total RNA in pancreas, hypothalamus and ovary were extracted and the amount of AR mRNA was quantitated by RT-PCR with single base mutated template as inner standard. Results: Serum concentrations of △4-A, TT, FT, Ins and C-P in ASR models were significantly higher than those in control group (P<0. 05, P<0. 01). The expression of AR mRNA in pancreas, hypothalamus and ovary increased significantly in model rats as compared with control group (P<0. 05, P<0. 01). Conclusion:The elevated serum androgen levels in ASR model could enhance the expression of AR mRNA in pancreas, hypothalamus and ovary, and then induced hyperinsulinemia and anovulation.