转化生长因子β受体Ⅱ在大鼠实验性隐睾中的表达及与生精细胞凋亡的关系

The Expression of Transforming Growth Factor β Receptor Ⅱ in Experimental Cryptorchidism and Apoptosis in Spermatogenic Cells in Rat

目的:探讨转化生长因子β受体Ⅱ(TGFβ-RⅡ)与实验性隐睾诱导的生精细胞凋亡的关系。方法:建立大鼠单侧隐睾动物模型,SABC法检测TGFβ-RⅡ在隐睾及对照侧睾丸组织内的分布及表达,TUNEL法检测凋亡生精细胞。结果:术后不同时间组隐睾侧曲细精管TGFβ-RⅡ表达均低于对照侧(P<0.01)。术后隐睾侧细胞凋亡数量较对照侧明显增加(P<0.01)。结论:TGFβ可能通过TGFβ-RⅡ介导的信号转导过程在睾丸生精细胞凋亡过程中发挥重要的调控作用。

Objective:To observe the expression of transforming growth factor beta receptor Ⅱ (TGFβ-R Ⅱ) in experimental cryptorchidism and apoptosis in spermatogenic cells in rat. Methods: The apoptosis of spermatogenic cells was detected by means of TUNEL method (terminal deoxynucleotidyl transferase -mediated dUTP nick end lableling method) and the TGFβ-R I expression was observed with immunohis tochemistry SABC methods. Results:There was significant increase in the expression of TGFβ-R Ⅱ in unilateral undescended testes (UUTs) was compared with that in contralateral descended testes (CDTs) (P<0. 01). However, there was a significant and time-dependent increase in- the mean apoptotic index in UUTs than in CDTs in all groups. Conclusion:TGFβ-R Ⅱmay play an important role in spermatogenic cell apop tosis.