检测猪流行性腹泻病毒的R-PCR方法的建立

Detection of porcine epidemic diarrhea virus by RT-PCR

根据猪流行性腹泻病毒 (PEDV)的N基因自行设计和合成了一对可扩增长度为 641bp目的片段的引物 ,成功地建立了检测的猪流行性腹泻病毒的RT PCR方法。对猪轮状病毒 (PRV)、猪传染性胃肠炎病毒 (TGEV)的RT PCR检测结果均呈阴性。对PEDV JS株的RT PCR产物的序列分析表明 ,与CV777株的同源性为 97 3 %。

A PCR assay for rapid and sensitive detection of porcine epidemic diarrhea virus was developed.The PCR primers based on the N gene of porcine epidemic diarrhea virus could extend a 641 bp PCR product.Using the same pairs,no PCR product was detected from porcine rotavirus and transmissible gastroenteritis virus.The sequence homology of RT-PCR products of PEDV-JS strain and CV777 strain was 97.3%.