摘要
嫣红蔓带芽嫩茎及茎尖的最适初代培养基是1/2MS+BA1.0mg/L+NAA0.05mg/L,完全的MS配方易导致玻璃化;在继代培养时,交替采用增殖培养基1/2MS+BA0.3mg/L+NAA0~0.01mg/L和增高培养基1/2MS+BA0.1~0.2mg/L,这样便能保证既有较高的增殖率,繁殖的芽苗又比较长;嫣红蔓芽苗的最佳生根培养基是1/2MS+IBA0.01mg/L。
Tissue culture was made with the stem-segments and shoot tips of Hypoestes phyllostachya as explants. The optimum media were 1/2 MS + BA 1.0 mg/L + NAA 0.05 mg/L for primary culture, 1/2MS + BA 0.3 mg/L + NAA 0~0.01 mg/L for multiplication and 1/2 MS + BA 0.1~0.2 mg/L for shoot growth. The best rooting medium was 1/2 MS + IBA 0.01 mg/L.
出处
《西南农业大学学报(自然科学版)》
CSCD
北大核心
2004年第1期61-63,共3页
Journal of Southwest Agricultural University
关键词
嫣红蔓
离体繁殖
培养基
Hypoestes phyllostachya
in vitro propagation
culture medium