D16S539等5个短串联重复序列基因座遗传多态性分析

Genetic polymorphism analysis of five short tandem repeat loci in Henan

目的 探讨河南汉族群体 D16S53 9、D7S82 0、D13 S3 17、D8S1179和 D2 1S11等 5个基因座基因频率 ,获得群体遗传学数据。方法 随机抽取 2 0 9名河南汉族群体无血缘关系个体的静脉血 ,柠檬酸抗凝剂抗凝 ,酚 -氯仿法提取 DNA ,应用 PCR技术扩增上述 5个 STR基因座 ,聚丙烯酰胺凝胶垂直板电泳分型。结果 2 0 9名个体中 D16S53 9、D7S82 0、D13 S3 17、D8S1179和 D2 1S11等 5个基因座分别检出 7、8、8、10、15个等位基因 ,基因型分布符合 Hardy-Weinberg平衡。 5个基因座的杂合度分别为 0 .790 7、0 .80 56、0 .7914、0 .82 3 9、0 .80 46,多态信息含量分别为 0 .7712、0 .780 1、0 .7694、0 .80 0 9、0 .7816,个人识别能力在 0 .9416、0 .93 67、0 .9511、0 .9546、0 .93 0 8,非父排除概率分别为 0 .610 6、0 .6179、0 .614 5、0 .70 2 2、0 .6712。结论 5个 STR基因座具有高度多态性遗传标记特征 。

ObjectiveTo study allele frequency distributions of Ha n population in Henan area. MethodsThe polymerase chain reacti on (PCR) was used. Five STR systems were amplified on DNA samples, which were ex tracted from 209 unrelated individuals ACD blood by phenol/chloro form method. The PCR products were analyzed by PAG vertical electrophoresis.ResultsAmong 209 individual, 7, 8, 8, 10, 15 alleles were found at D16S539, D7S 820, D13S317, D8S1179 and D21S11 respectively. No deviations from Hardy-Weinber g equilibrium were observed. The heterozygosities observed were 0.7907,0.8056, 0.7914,0.8239 and 0.8046 for D16S539, D7S820, D13S317, D8S1179 and D21S11 respe ctively.The polymorphism information content were 0.7712,0.7801,0.7694,0.800 9 and 0.7816 respectively. The discrimination proability were 0.9416,0.9367,0 .9511,0.9546 and 0.9308 respectively. The exclusion power were 0.6106,0.6179, 0.6145,0.7022 and 0.6712 respectively. ConclusionAll of the five loci in this study were useful marke rs for genetics study and the forensic science practice.