摘要
对从土壤中分离到的58株放线菌进行了皿内拮抗试验及利用几丁质能力测定,在此基础上筛选出了F104,F1052株产几丁质酶的菌株,并测定了其对棉花枯、黄萎菌的离体和活体抑制作用。皿内观察发现,F104和F105均可寄生于棉花黄萎菌,F105还可寄生于棉花枯萎菌。平皿扩散试验结果发现,F104和F105的培养滤液无论经高温处理与否,均能使棉花黄萎菌呈现畸形菌丝,而经高温处理的F104还可使棉花枯萎菌表现为畸形菌丝。抑制靶标致病菌寄主活体定殖作用的研究结果表明,F104和F105处理均表现出不同程度的超前接种防治黄萎病的作用,超前接种F105的间隔期与棉苗中枯萎菌的检出率呈负相关。
The antagonistic effects and the chitinolytic ablity of the 58 isolates of the actinomyces were tested in vitro and 2 selected isolates F104 and F105 with chitinolytic ablity were screen for suppression of F.oxysporum f.sp.vasinfectum and V.dahliae.The mycelium of V.dahliae growth malformed in PDA diffusion test with the culture filtration of isolates F104 and F105.An effective control result to F.oxysporum f.sp.vasinfectum and V.dahliae were obtained when pre-inoculation of cotton seedling with isolates F104 and F105 in greenhouse.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2003年第6期63-65,共3页
Journal of Northwest A&F University(Natural Science Edition)
基金
农业部农业结构调整重大技术研究项目(2002-08-03A)
西北农林科技大学重点科研项目
关键词
棉花枯萎菌
棉花黄萎菌
几丁质降解菌
生物防治
F.oxysporum f.sp.vasinfectum
Verticillium dahliae
chitinolytic actinomycetes
biological control
