RP-HPLC法测定原料药中头孢拉定的含量

Determination of Cefradin by RP-HPLC

利用反相液相色谱法(RP-HPLC)直接测定原料药中头孢拉定的含量.采用YQG-ODS色谱柱,以V(CH3OH)∶V(4%HAc-NaAc)=3∶7的溶剂作流动相,于λ=262nm处紫外检测,以峰面积外标法定量.头孢拉定在0.027～0.29g·L-1浓度范围内具有良好的线性关系,回归方程为Y(μV·s)=1.88×107X(g·L-1)+20876,r=0.9983,平均回收率为99.7%,RSD为0.57%(n=3).

A RP-HPLC method for the determination of cefradin in raw drug was established.A column of YQG-ODS was used with a mobile phase of V(CH3OH)∶V(4%HAc-NaAc)=3∶7 solution.The UV detector was set at 262 nm.The external standard method was used.There was a good linear correlation in the range of 0.027～0.29 g·L-1,Y(μV·s)=1.88×107X(g·L-1)+20 876,r=0.998 3.The average recovery was 99.7%,RSD was 0.57%(n=3).The elapsed time of a chromatographic analysis was 12 min.The method is rapid,accurate and reliable.