生物质谱作为SNP分型检测方法的研究

Study on Single Nucleotide Polymorphisms Genotyping by Bio-Mass Spectrometry

利用改良的 Miller法从人血中提取人类基因组 DNA。采用聚合酶链式反应 ( Polymerase chain reac-tion,PCR) ,对含有单核苷酸多态性 ( SNP)点的 DNA片段进行扩增 ,然后用 CIP和 Eox I去除掉 PCR体系中剩余的脱氧核糖核苷三磷酸 ( d NTP)和引物 ,最后用单碱基延伸反应获得 SNP位点处碱基类型。用基质辅助激光解吸电离 -飞行时间质谱 ( MALDI-TOFMS)或电喷雾电离 -四极杆飞行时间质谱 ( ESI-Qq TOFMS)检测延伸产物与未延伸引物间的分子量差异 ,确定该点处碱基。

The deoxyribonucleic acid (DNA) in the human genome was extracted from the human blood by the improved Miller method. The DNA fragments including SNP loci were amplified, and the remaining dNTPs and primers were digested using CIP and Exo I. Then the base on the SNP loci was determined by the single base extension reaction. Finally, the base was identified from the molecular weight difference between the extension product and extension primer by bio mass spectrometry (MALDI TOFMS or ESI QqTOFMS). The advantages and disadvantages of the different bio mass spectrometry including MALDI TOFMS and ESI QqTOFMS used to measure SNP genotyping were discussed,and different bio mass spectrometry methods with the other current detecting methods used to detecting SNP genotyping was compared.