前列腺分泌蛋白94基因的克隆及序列测定

Clone and sequence analysis of prostate secretory protein of 94 amino acids

目的 :作为构建前列腺分泌蛋白 94 (PSP94 )分泌型表达质粒的基础。方法 :从正常前列腺组织中钓取PSP94cDNA ,构建重组质粒pUC19-PSP94 ,转化后提取目的基因并进行测序。结果 :( 1)构建了pUC19-PSP94的重组克隆质粒 ;( 2 )PCR扩增获得了 2 80bp的PSP94成熟蛋白基因 ;( 3 )测定了PSP94的基因序列。结论 :所构建的pUC19-PSP94重组质粒可成功地克隆PSP94蛋白基因。

AIM: To construct recombinant human prostate secretory protein of 94 amino acids(PSP94) expression vector. METHODS: The PSP94 cDNA was obtained from normal prostate tissue, and recombinant plasmid pUC19-PSP94 was constructed. The target gene was identified and sequenced. RESULTS: 1) The recombinant plasmid pUC19-PSP94 was constructed; 2) The mature PSP94 protein gene of 280bp was acquired by PCR; 3) The gene sequence of PSP94 was identified.CONCLUSION: The constructed plasmid pUC19-PSP94 could to be clone PSP94 successfully.