摘要
目的与方法 :体外培养大鼠系膜细胞 ,以脂多糖 (lipopolysaccharide ,LPS)刺激后 ,分别加入补肾活血泄浊汤有效成分川芎嗪、大黄素、灵芝多糖 ,应用Northern杂交技术 ,观察上述几种中药有效成分对系膜细胞IL - 6基因表达的影响 ,以探讨补肾活血泄浊汤治疗肾小球肾炎的机制。结果 :川芎嗪、大黄素、灵芝多糖均可抑制IL - 6基因的表达 ,以大黄素最为显著。结论 :结果提示补肾活血泄浊汤可能通过抑制IL - 6基因的表达 ,从而减轻系膜细胞增生。
AIM and METHODS: Cultured mesangial cells were stimulated by lipopolysaccharide(LPS), then were treated with some valid components in BUSHENHUOXUEXIEZHUOTANG: emodin, ligustrazine and ganoderma polysaccharide. The effects of these components on interleukin-6(IL-6) mRNA expression in mesangial cells were identified by Northern hybridization. RESULTS: Emodin, ligustrazine and ganoderma polysaccharide inhibited LPS-induced IL-6 gene expression in mesangial cells.CONCLUSION: BUSHENHUOXUEXIEZHUOTANG may relieve mesangial cells proliferation by inhibiting IL-6 mRNA expression.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2001年第1期23-24,共2页
Chinese Journal of Pathophysiology
基金
国家教委博士点科研基金资助课题
关键词
中草药
大鼠
杂交
RNA
基因表达
Drugs, chinese herbal
Rats
Hybridization
RNA
Gene expression
