贲门癌切除标本切缘残留癌的流式细胞分析及其与预后的关系

FLOW CYTOMETRIC ANALYSIS OF RESIDUAL TUMOR OF THE RESECTED MARGINS (RTRM) IN CARDIAC CANCER AND THE RELATIONSHIP WITH PROGNOSIS

研究目的探讨贲门癌切除术切缘残癌细胞的DNA倍体类与患者预后的关系以及残癌细胞的克隆起源。研究设计病例对照研究研究对象 1981～1985年间手术切除的贲门癌标本共40例,患者均经5年随访。处理方法样品制成单细胞悬液,经过DNA染色,用FACS-Δ20型流式细胞计检测。每例样品需测2万个细胞。结果以组方图和数字表的形式显示并打印。测定结果 40例残癌中,2倍体(2C)16例,占40％;异倍体(AN)24例,占60％。2C患者的3年及5年生存率显著高于AN患者(P<0.05)。残癌的倍体类型与主体癌之间无显著性差异(P>0.05)。结论 DNA倍体是判断残癌患者预后的重要生物学指标;残癌细胞起源于与主体癌相同的DNA干系细胞群。

Objective To search for the relationship between cellular DNA ploid patterns of RTRM in cardiac cancer and the patients pronosis and the clonal origin of RTRM cells. Design Case—control ressearch. Patients and Other Participants Fourty cases of risected specimens comens coming from the patients suffering from cardiac cancer during from 1981 to 1985; All of patients have been followed—up for 5 years. Interventions Samples were made into suspension liquid with single and were stained for nuclear DNA. At least 20000 cells were examined by flow cytometry (FCM) in each specimens. Measurement and Results Of the 40 cases, 16 (40%) cases were diploid (2C) DNA patterns and 24 (60%) were aneuploidy(AN). The rate of 3, 5-year-survival in patients with 2C was significantely higher than that in patients with AN (P<0.05). There was no significantely difference in DNA ploid patterns between RTRM and correspounding main tumors. Conclusion DNA ploid patterns is a significant prognostic indicator of patients with RTRM; RTRM cells originete from the same DNA stem cell populations as the correspounding primary tumors.