服药动物排泄物中微量砷的微波消解-氢化物发生原子荧光法测定

Determination of Arsenic in the Excrement Samples of Medicine-Taking Animal by HG-AFS with Microwave Digestion

采用微波消解的样品预处理技术和氢化物发生 -原子荧光法 ,测定了服药 (安宫牛黄丸、雄黄、二硫化二砷 )实验动物大鼠不同时间的粪便排泄物中砷含量 ,初步研究了砷的排泄情况 ;通过实验 ,确定了微波消解样品的条件 ,优化了仪器的最佳工作参数 ;以硝酸和高氯酸混合液作消解液 ,硫脲和抗坏血酸为混合预还原掩蔽剂 ,4.0 %硼氢化钾溶液作还原剂 ,测定结果满意 ;表明该法快速、简便、准确 ;砷的检出限为0.06μg/L,线性范围为0～20μg/L;为安宫牛黄丸的减毒机理、药效和毒性的科学评价提供了一定的依据。

A method based on microwave digestion—hydride generation atomic fluorescence spectrometry for the determination of arsenic(As)in the excrement samples of medicine-taking animal was established.The medicines taken were Angongniuhuangwan,Realgar and As 2 S 2 ,respectively.The experimental condiˉtions and the instrumental parameters were optimized.The distribution of arsenic in different time was also investigated.The detection limit for As was0.06μg/L and the linear range of As was0～20μg/L(r=0.9993).The method is accurate,rapid and convenient.Moreover,it can provide certain basis for the investigation of the toxicity-reducing mechanism of Angongniuhuangwan and the scientific evaluation of the medicinal and toxicity of Angongniuhuangwan.