摘要
基于共聚焦光学结构 ,以发射波长532nm的二极管泵浦固体激光器为激发光源研制了一小型荧光检测器 ;以罗丹明B为荧光试剂 ,柱上检测模式评价了该检测体系 ,焦宁Y为背景荧光探针间接检测了无机金属离子 ,结果表明该系统具有体积小、成本低、高灵敏度和易操作等优点。
Aportable laser-induced fluorescence detector based on confocal configuration is described,usˉing a laser-diode double-pumped solid-state laser(LD-DPSSL)as the excitation source.On-colˉumn detection modes with Rhodamine B were employed for the evaluation of the LIF-D system.Indirect fluorescence detection of mental cations using Pyronine Yas the background probe was performed.The reˉsults showthat this systemhas the advantages of small size,lowcost,high sensitivity and easy operation.
出处
《分析测试学报》
CAS
CSCD
北大核心
2004年第1期91-93,共3页
Journal of Instrumental Analysis
基金
科技部"十五"科学仪器攻关资助项目 (011806)