激光二极管泵浦激光诱导荧光检测装置的研究

A Laser-induced Fluorescence Detection System with a Laser Diode Double-pumped Solid State Laser as the Excitation Source

基于共聚焦光学结构 ,以发射波长532nm的二极管泵浦固体激光器为激发光源研制了一小型荧光检测器 ;以罗丹明B为荧光试剂 ,柱上检测模式评价了该检测体系 ,焦宁Y为背景荧光探针间接检测了无机金属离子 ,结果表明该系统具有体积小、成本低、高灵敏度和易操作等优点。

Aportable laser-induced fluorescence detector based on confocal configuration is described,usˉing a laser-diode double-pumped solid-state laser(LD-DPSSL)as the excitation source.On-colˉumn detection modes with Rhodamine B were employed for the evaluation of the LIF-D system.Indirect fluorescence detection of mental cations using Pyronine Yas the background probe was performed.The reˉsults showthat this systemhas the advantages of small size,lowcost,high sensitivity and easy operation.