尼莫地平对大白鼠脑梗死区钙离子活动的影响

Effect of nimodipine on calcium ion in cerebral infarct areas in rats

雄性大白鼠24只,随机分成3组各8只。A组为对照组,在大脑中动脉凝闭(MCAO)前30min静注蒸馏水;B组在MCAO前30min静注尼莫地平0.6mg/kg;C组在MCAO后40min静注尼莫地平1mg/kg;用钙离子选择性微电极观察脑梗死区细胞外Ca^(2+)变化。结果:B组脑梗死区细胞外Ca^(2+)下降值较小,A组下降值较大(P<0.01);C组给药前后自身对照脑梗死区细胞外Ca^(2+)明显回升(P<0.01)。实验表明,在MCAO前或后给药,尼莫地平对脑梗死区细胞外Ca^(2+)流动均有阻滞作用。

Twenty four rats were equally divided into 3 groups. Distilled water was injected iv 30 min before middle cerebral artery occlusion (MCAO) in group A. Nimodipine 0.6 mg/kg was injected iv 30 mia before MCAO in group B and 1 mg/kg 40 min after MCAO in group C. Changes of Ca2+ in infarct area were detected with the aid of ion-selective microelectrodes (ISMs). The results showed that the concentration of extracellular Ca2+ in infarct areas of group B decreased significantly less than that of group A (P<0.01). The concentration of extracellular Ca2+ in group C increased after nimodipine (P<0.01). Nimodipine given before or after MCAO ir-hibited the passage of extracellular Ca2+ into the brain cell in infarct areas.