食品中转基因成分的PCR筛选检测方法

The Study on PCR Screening Methods for Detecting GMO in Food

对 3种常用食品中转基因成分筛选检测方法的灵敏度及特异性进行了研究。试验结果表明 ,35S筛选方法的理论检测低限达 1 5copies,NPTⅡ筛选方法的理论检测低限达 5 0copies,NOS筛选方法的理论检测低限达 1 5 0copies。在不含转基因成分的材料中均未检测到以上 3个片断。

This study was focused on the theoretical detection limit of three normally applied PCR screening methods in GMO detection.The results show that 15 copies are the theoretical detection limits of 35S screening methods 50 and 150copies are necessary for NPTⅡ gene and NOS terminator detection.These 3 target DNA fragments were detected in the non-GMO samples.