共聚焦激光扫描显微镜活体观测川芎嗪和去甲基肾上腺素对生理状态下家兔大脑皮质内微循环的影响

Observation of the Cerebral Cortex Microcirculation of the Live Rabbit Treated with Ligustrazine and Noradrenalin by Confocal Laser Scanning Microscopy

目的 :用共聚焦激光断层扫描显微镜活体观测川芎嗪和去甲基肾上腺素对正常家兔大脑皮质内微循环的影响。方法 :在开放颅窗的家兔动物模型上 ,荧光素标记血浆 ,罗丹明 6G标记WBC ,用共聚焦激光扫描显微镜活体观测正常状态下家兔大脑皮质内 3 0 0 μm深处微循环 ,并经图像分析系统测量数据 ,用SAS软件包进行统计学分析。结果 :川芎嗪对大脑皮质内动脉的作用与去甲基肾上腺素相似 ,认为川芎嗪既可兴奋β受体 ,也可兴奋α受体。去甲基肾上腺素和川芎嗪使平均口径为 12 1.5 6μm ,60 .0 0 μm的动脉血管运动 ,而缘流在血管运动时是保持不变的 ;在毛细血管处可引起不同程度的血管舒缩运动。结论 :在共聚焦激光扫描显微镜活体观测正常状态下家兔大脑皮质内3 0 0 μm深处微循环 ,用去甲基肾上腺素和川芎嗪使平均口径为 12 1.5 6μm ,60 .0 0 μm的动脉血管运动时 ,血液缘流是保持不变的 ;

Objective: To observe the cerebral cortex microcirculation of the live rabbit treated with ligustrazine and noradrenalin by confocal laser scanning microscopy.Method: Confocal Laser scanning microscopy was used in a rabbit opening cranial window preparation in order to observe the microcirculation from the surface to 300 μm depth within the cerebral cortex of normal state, the plasma was labeled by fluorescein and WBC was labeled by rhodamine 6G. statistic data was analysed by SAS soft.Results: We found that the effect of Ligustrazine to the microcirculation within the cerebral cortex of normal state was the same as noradrenaline.We thought that Ligustrazine both excited β-receptor and excited α-receptor. Noradrenaline and Ligustrazine cause that the diameter of artery was 121.56μm,60.00μm to produce the movement of diastole and systole, and the thickness of edge-flowing blood in the vessels remains along with the movement. Noradrenaline and Ligustrazine cause the movement of diastole and systole on the microvessel. Conclusion: Confocal Laser scanning microscopy was used in a rabbit opening cranial window preparation in order to observe the microcirculation from the surface to 300 μm depth within the cerebral cortex of normal state,we found while Noradrenaline and Ligustrazine cause that the diameter of artery was 121.56 μm,60.00 μm to produce the movement of diastole and systole,and the thickness of edge-flowing blood in the vessels remains along with the movement.Noradrenaline and Ligustrazine can cause the movement of diastole and systole on the microvessel.