丹参对兔重症急性胰腺炎早期中性粒细胞与内皮细胞粘附机制的研究

Effect of Salvia Miltiorrhiza on Inhibiting Polymorphonuclear Leukocyte and Endothelial Cells Adhesion in Rabbits with Severe Acute Pancreatitis of Early Stage

目的 :研究丹参对兔重症急性胰腺炎 (SAP)早期中性粒细胞 (PMN)与内皮细胞 (EC)粘附作用及对血浆肿瘤坏死因子 α(TNF α)的影响。方法 :将日本大耳兔 2 4只建立兔SAP模型 ,随机分为丹参治疗组和对照组。分别于术前 ,术后即刻、2、4、8、16h ,取血分离获得PMN。与体外培养的兔血管EC作用测定PMN EC粘附率 ,并测定各时点血浆TNF α值。结果 :两组术后即刻粘附率均升高 ,对照组术后 2hTNF α开始升高 ,8h达高峰 ,16h仍高于正常 ;治疗组 2、4、8、16h各时点的TNF α的值小于对照组 (P <0 .0 5 )。结论 :丹参能降低SAP血浆TNF α的水平 ,进而抑制PMN EC粘附 。

Objective:To study the effect of salvia miltiorrhiza on the adhesion of polymorphonuclear leukocyte(PMN) and endothelial cells(EC) and on TNF α in rabbits with severe acute pancreatitis(SAP) of early stage. Methods: Twenty four Japan rabbits were induced SAP and divided randomly into control group and salvia miltiorrhiza treatment group. Blood was obtained respectively before operation, instantly after operation, 2 hours, 4 hours, 8 hours, 16 hours after operation. PMN was separated from the blood and interacted with rabbit's vascular EC cultured in vitro. The adhesion ratio of PMN and EC and expression of TNF α was measured. Results: After operation, the adhesion ratio of the two groups both rose. In the control group TNF α began to rise 2 hours after operation, reached the peak 8 hours after the operation, and was above normal 16 hours after operation. In the treatment group 2 hours, 4 hours, 8 hours, 16 hours after operation, TNF α was lower than the control group (P<0.05). Conclusion: Saliva miltiorrhiza can lower the TNF α level of SAP of early stage, thus inhibit the adhesion of PMN and EC, improve the microcirculation and ease the tissue damage.