摘要
目的 通过建立大鼠自体静脉移植模型探讨静脉移植后再狭窄机制及药物对其影响以期减少再狭窄率,为临床用药提供理论依据。方法 将大鼠自体颈静脉外侧分支间置移植于颈总动脉,术前1周开始分组给口服药卡托普利[100mg/(kg·d)]、阿司匹林[40mg/(kg·d)]及联合用药至术后2周。应用病理形态学方法通过计算机图像定量分析,观察自体移植后静脉内膜平滑肌细胞增殖的情况及药物对其影响。应用增殖细胞核抗原(PCNA)免疫组织化学方法,观察其对自体移植静脉血管平滑肌细胞(VSMC)中PCNA表达的变化。结果 卡托普利组及卡托普利加阿司匹林组与对照组相比移植静脉内膜增生程度及静脉内膜PCNA阳性细胞指数差异有显著性(P<0.05);阿司匹林组与对照组相比移植静脉内膜增生受到一定的抑制,但无显著差异。而阿司匹林组PCNA阳性细胞指数无显著差异。结论 卡托普利可明显抑制静脉移植后平滑肌细胞反应性增生。而阿司匹林无抑制作用。
Objective To assess the mechanism of restenosis after vein graft and the effect of drugs on intimal hyperplasia of vein autografts by transplanting the autologous internal jugular vein to common carotid artery in rats. Methods The treated animals were given drugs daily from one week before operation to two weeks after operation. The grafts were got to analyse intimal hyperplasia by pathological morphology and PCNA immunohistochemistry. The intimal hyperplasia was measured with a computerized image analysis system. Results There was significant difference(P<0.05) in the degree of intimal hyperplasia and the number of PCNA positive cells between the control and the Capton group,but there was no significant difference(P> 0.05) between the control and the Aspirin group. Conclusion Captopril can inhibit intimal hyperplasia after vein autograft while Aspirin can not. It provide a clue for preventing restenosis with drugs after vein graft.
出处
《中国心血管病研究》
CAS
2004年第2期149-151,共3页
Chinese Journal of Cardiovascular Research
基金
国家自然科学基金资助项目
