PPD对人PBMC的HIV复制和细胞因子产生的影响

The effect of PPD on HIV replication and cytokines production in human PBMC

目的 :明确结核菌素纯蛋白衍化物 (PPD)对PBMC内HIV 1复制的作用及其对细胞因子分泌的影响。方法 :HIV 1SF 33毒株分别感染结核菌素试验阳性 (PPD(+) )和阴性 (PPD(- ) )健康人的PBMC后 ,与PPD共孵育 72小时 ,用ELISA方法检测上清中HIV 1p2 4抗原、TNF α和IL 10的含量。结果 :在PPD(+)的健康人 ,PPD能显著诱导PBMC内HIV 1的复制 ,促进HIV 1感染的PBMC分泌TNF α,同时抑制IL 10的分泌。在PPD(- )的健康人 ,未见到PPD显著诱导PBMC内HIV 1的复制。结论 :在我国PPD(+)的健康人 ,PPD促进HIV 1感染的PBMC分泌TNF α ,并抑制IL 10的分泌 ,导致HIV 1复制增加。

Objective:To identify the effect of PPD on HIV replication and the production of cytokines in peripheral blood mononuclear cell(PBMC).Methods:PBMC from PPD(+) healthy persons and PPD(-) persons were infected with HIV-1 SF-33, respectively, then incubated with PPD for 72 hours. HIV-1 p24, TNF-α and IL-10 in the supernatant were detected by ELISA.Results:PPD significantly increased the replication of HIV in PBMC from PPD(+) healthy donors rather than from PPD(-) healthy persons. PPD induced the secretion of TNF-α but inhibited IL-10 production in HIV-1 infected PBMC.Conclusion:PPD increased the replication of HIV and the production of TNF-α and inhibited IL-10 production in HIV-1 infected PBMC from PPD(+) healthy person.