摘要
目的 建立人可溶性CD14双抗体夹心ELISA检测体系。方法 用抗人CD14单抗为捕获抗体 ,加入待测抗原 ,第二抗体为兔抗人CD14多克隆抗体 ,最后加入辣根过氧化物酶标记的羊抗兔IgG。结果 本检测体系灵敏度高 ,可达 5ng ml,特异性强 ,测量范围 5～ 12 0ng ml,重复性好 ,各项技术指标与国外同类产品相当。结论 本检测体系能够检测人体液中和细胞培养上清中sCD14 ,适用于临床及基础研究。
Objective To establish a sandwich ELISA for the determination of soluble CD14 in human body fluid. Methods An anti-CD14 monoclonal antibody was coated on polystyrene microtiter plates; then samples for detection were added to the wells; followed by anti-human CD14 polyclonal antibody prepared from rabbit; at last, addition of HRP-goat anti-rabbit IgG completed the sandwich ELISA. Results The specificity of this sCD14 ELISA is high, the lowest detectable level is 5ng/ml, the range of detection is 5ng/ml～120ng/ml. Conclusion This sCD14 ELISA is applicable for research and observation in clinical.
出处
《中国实验诊断学》
2004年第1期34-37,共4页
Chinese Journal of Laboratory Diagnosis
基金
广东省自然科学基金资助 ( 0 0 10 5 8)
