肿瘤耐药蛋白在舌鳞癌中的表达及意义

Expression and Implication of Pgp, MRP, LRP, GST-π, TopoⅡα in Tongue Squamous Cell Carcinoma

目的 检测P_糖蛋白 (Pgp) ,多药耐药相关蛋白 (MRP) ,肺耐药相关蛋白 (LRP) ,谷胱苷肽_S转移酶 (GST_π)及DNA拓扑异构酶Ⅱα(TopoⅡα)在舌鳞癌组织中的表达水平及与化疗疗效的关系。方法 采用卡铂、平阳霉素和氨甲喋呤化疗方案对 4 0例舌鳞癌患者进行化疗 ,分别于化疗前和化疗后用免疫组织化学LsAB法检测Pgp ,MRP ,LRP ,GST_π ,TopoⅡα在 80例舌鳞癌组织中的表达 ,其中 4 0例为化疗前 ,4 0例为化疗后。结果 在检测的舌癌组织中 ,化疗前Pgp ,MRP ,LRP ,GST_π ,TopoⅡα的阳性表达率分别为 4 7 5 % ,5 0 % ,35 % ,4 5 % ,82 5 % ,它们的表达与临床病理无关 (P >0 0 5 ) ;Pgp ,MRP化疗前后水平上升 (P <0 0 5 ) ;Pgp,MRP与化疗耐药效果有关 (P <0 0 5 ) ;共表达现象普遍 ,Pgp ,MRP ,GST_π及MRP ,GST_π联合表达率在化疗无效者中为 4 0 %和 5 0 % ,而在化疗有效者中为 0。结论 Pgp ,MRP 。

Objective To explore the correlation of chemotherapy efficacy in tongue squamous cell carcinoma(SCC) with expression level of P-glycoprotein(Pgp), multidrug resistance-associated protein (MRP), lung resistance-related protein (LRP), glutathiones-tranferase (GST-π), DNA topo-isomerase Ⅱα (TopoⅡα).Methods The expression patterns of Pgp, MRP, LRP, GST-π and TopoⅡα in 40 patients (pre and post-chemotherapy, respectively) with tongue SCC were examined by immunohistochemically labelled streptavidin bioein method (LsAB).Results The expression ratios of Pgp, MRP, LRP, GST-π and TopoⅡα in pre-chemotherapy cases were 47 5%, 50%, 35%, 45%, 82 5%, respectively. No relations between expression of Pgp, MRP, LRP, GST-π, TopoⅡα and clinic indexes were established (P>0 05). Expression ratios of Pgp, MRP in post-chemotherapy cases were higher than that in pre-chemotherapy cases (P<0 05). Expression of Pgp and MRP showed relevance with drug resistance (P<0 05). The co-expression was common, the ratios of co-expression of Pgp, MRP, GST-π and MRP, GST-π in chemotherapy non-responders were 40% and 50%, respectively, but 0 in responders. Conclusion The intrinsic multidrug resistance of tongue SCC is relevant to the effects of Pgp, MRP, GST-π.