功能矫形前伸下颌后大鼠髁突软骨细胞睾酮的分布变化规律

Expression of Testosterone in the Rapid Growing Rat Condylar Cartilage and the Expression Changes after the Function Mandibular Advacement

目的 研究快速生长期大鼠髁突软骨细胞睾酮的表达 ,并探讨功能矫形对髁突软骨细胞睾酮表达的影响。方法 实验组戴自制模拟的功能矫治器引导大鼠下颌前伸 ,在实验第 3天、1、2、3及 4周处死大鼠 ,应用免疫组织化学SP法检测睾酮在髁突软骨细胞上的表达特点。结果 ①实验组、对照组大鼠髁突软骨细胞各层都有睾酮的阳性免疫染色 ,大鼠各层细胞中以成熟层及移行层阳性强度较高 ,明显高于生发层及过渡层 ,其中成熟层染色强度最高 ;②随着大鼠的青春期生长发育 ,髁突软骨细胞各层睾酮染色强度出现不同的变化特点 ;③实验第 2及第3周组大鼠髁突成熟层软骨细胞睾酮染色强度明显高于对照各组 ,差异具有统计学意义 (P <0 0 5 )。结论 睾酮参与了大鼠髁突软骨细胞成熟的调控 ,并介导了功能矫形的机械刺激作用 ,使髁突软骨细胞的增生分化功能增强 。

Objective To investigate the expressions of Testosterone in the condylar cartilage of rapid growing rats.Methods The mimic functional appliances were used in experiment groups and rats were killed after 3 days , 1week, 2weeks, 3weeks and 4weeks. Immunohistochemical technique was used to detect the expression of T.Results T was expressed throughout the condylar cartilage. The Highest expressions were in mature layers of both experiment and control groups. Along with the maturation, the expressions of T in the condylar cartilage were changed respectively, and the immunostain levels in the mature layer of experiment groups 2 and 3 weeks were higher than those of control groups (P<0 05).Conclusion ①T is a member of cartilage growth and development control system. ②The orthopedic mechanism of functional appliance may be conducted by T.