黄芩对牙髓卟啉单胞菌产丁酸影响的实验研究

The Effect of Redix Scutellariae on Butyrate of Porphyromonas endodontalis in vitro

目的 通过研究黄芩对牙髓卟啉单胞菌生长代谢的影响 ,探讨黄芩治疗牙髓根尖周病的作用机制。方法 采用试管两倍稀释法测定黄芩对牙髓卟啉单胞菌 (P .e)的最小抑菌浓度 (MIC)。采用高效液相色谱仪测定低于MIC4个浓度的黄芩对P .e产丁酸的影响。结果 黄芩对P .e的MIC为 10 0mg L。随着药物浓度升高 ,P .e产丁酸量降低 ,其值分别为 (3 5 2 7± 0 0 0 9)mg L ,(3 0 4 8± 0 0 0 5 )mg L ,(2 4 90± 0 0 11)mg L ,(2 2 0 9± 0 0 16 )mg L。结论 黄芩对P .e生长。

Objective To study the effect of Radix Scutellariae on the growth, metabolism of Porphyromonas endodontalis(P.e), as a preparation for studying the mechanism of Radix Scutellariae in treating pulp and periapical diseases.Methods P.e was chosen as the experimental bacteria. Radix Scutellariae was extracted by means of reflux with 80% ethanol. The value of MIC of Radix Scutellariae was measured by minute amount serial dilusion test, and the production of butyrate was measured by high liquid chromatograph(HPLC).Results Radix Scutellariae could inhibit the growth of P.e, of which the MIC was 100 mg/L. Following the increase in concentration of Radix Scutellariae, the amount of butyrate decreased to (3 527±0 009) mg/L, (3 048±0 005) mg/L, (2 490±0 011) mg/L, (2 209±0 016) mg/L, respectively(P<0 05).Conclusion Radix Scutellariae could inhibit the growth and metabolism of P.e and might be an effective agent in treating pulp and periapical diseases.