碱性成纤维细胞生长因子对犬牙周成纤维细胞样细胞增殖影响的体内研究

The Effect of bFGF on Proliferation of Periodontal Fibroblast-like Cells of Dogs

目的 观察碱性成纤维细胞生长因子 (bFGF)对犬牙周成纤维细胞样细胞增殖的影响。方法 选用 4只成年杂种犬 ,将其上颌两侧的前磨牙 (P1_3)、第一磨牙 (M1 )、下颌两侧的前磨牙 (P2_4 )、第一磨牙 (M1 )作为实验牙。在每个象限 4个实验牙的近中根颊侧人工制备“U”形骨缺损 ,并设计为 4组 ,即对照组、bFGF组、ePTFE组、bFGF +ePTFE组。每周手术 1次 ,每次手术做 1个象限的 4个牙位 ,连续 4次。完成 4次手术后 1周处死动物。动物处死前 1h注射尿嘧啶脱氧核苷 (BrdU) ,标本行免疫组化染色 ,显微镜下计数BrdU标记的成纤维细胞样细胞。结果 各组中BrdU+ 细胞数均于术后 2周最多 ,术后 3、4周显著减少。术后 1、2周 ,bFGF组和bFGF +ePTFE组的BrdU+ 细胞明显多于对照组和ePTFE组。结论 牙周术后 2周是牙周功能细胞增殖的活跃期 ,bFGF的局部应用可明显促进牙周愈合早期牙周功能细胞的增殖 。

Objective To evaluate the effects of basic fibroblast growth factor(bFGF) on proliferation of periodontal fibroblast-like cells in vivo.Methods A U-shaped osseous defect was produced on the buccal side of the mesial root. Four posterior teeth were conducted in four quadrants. Each quadrant included 4 groups: control, bFGF, expanded polytetrafluoroethylene(ePTFE) membrane, bFGF+ePTFE. Each time the 4 teeth sites in one quadrant were operated weekly and each dog experienced 4 times of operations. Bromodeoxyuridine(BrdU) was injected 1 hour prior to sacrificing the dogs at 4 weeks after first surgery. Immunohistochemical method was applied to count the BrdU-labeled fibroblast-like cells.Results The number of BrdU-labeled cells reached the maximum at the 2nd week among all groups and then, decreased with time. Both bFGF and bFGF+ePTFE treated group had significantly more BrdU++ cells than remained control or ePTFE groups (P<0 05) at 1st, 2nd weeks after surgery.Conclusion 2 weeks after periodontal surgery is active phase of proliferation of periodontal fibroblasts. bFGF enhances fibroblast proliferation in early periodontal wound healing, and in turn accelerate periodontal regeneration.