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光动力疗法对人膀胱癌细胞胞内游离钙的影响 被引量：4

Effect of Photodynamic Therapy on Free Ca^(2+) in Human Bladder Cancer Cells
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摘要 目的 :探讨光动力学作用后的膀胱癌细胞 (T -2 4和SCaBER)胞内钙离子浓度的变化。 方法 :采用MTT比色分析法判断光动力学对体外人膀胱癌T -2 4和SCaBER细胞的杀伤效应。同时应用激光共聚焦显微镜和Fluo -3 /AM探针技术测定光动力学作用后细胞内钙离子的浓度。 结果 :T -2 4和SCaBER细胞内钙离子有明显升高 ,与对照组比较差异有显著意义 (P <0 0 0 1)。 结论 ObjectiveTo investigate the changes in [Ca 2+ ]i in bladder cancer cells (T-24 and SCaBER cell)after photodynamic therapy(PDT )MethodsThe photodynamic cytotoxity of human bladder cancer cells(T- 24 and SCaBER) was studied by MTT assay.The change in [Ca 2+ ]i in bladder cancer cells following PDT was detected by means of a confocal laser scanning m icroscope using the Ca 2+ indicator Fluo-3/AM staining.ResultsIntracellular calcium concentration in T-24 cells and SCaBER cells increas ed markedly as compared with that in control cells( P <0 001).Con clusionIntracellsular calcium overload may play an important role in PDT induced cell death response processes.
作者 刘树硕 杨永华 裘顺安 王慎鸿 王泽时
机构地区 浙江中医学院附属医院泌尿外科
出处 《中国中西医结合外科杂志》 CAS 2004年第1期32-33,共2页 Chinese Journal of Surgery of Integrated Traditional and Western Medicine
基金 浙江省自然基金资助 ( 30 1 430 )
关键词 膀胱癌 游离钙 光动力疗法 叶绿素衍生物 激光共聚焦显微镜 bladder cancer chloropyll derivative,photodynamic t herapy,calcium overload,confocal laser scanning microscope
分类号 R737.14 [医药卫生—肿瘤]
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共引文献13

同被引文献17

  • 1曾娅莉,黄文芳,卢贤瑜.细胞凋亡时线粒体钙离子信号调节因素研究进展[J].国际检验医学杂志,2006,27(6):543-545. 被引量：21
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引证文献4

二级引证文献19

中国中西医结合外科杂志
2004年 第1期

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