高温高湿环境下猫颅脑火器伤后细菌生长特点

Bacterial growth characteristics in craniocerebral gunshot wound of cat in a hot and humid environment

目的 研究高温高湿环境下猫颅脑火器伤后创面及周围组织细菌生长特点。方法 杂种猫23只随机分为常温枪伤对照组(伤后立即取材，A组)、常温枪伤组(伤后6h取材，B组)、高温高湿枪伤组(伤后6h取材，C组)和空白对照组(D组)，分别取创面及周围组织进行细菌培养并计数。结果 A、B、C组其创面组织、伤道壁5mm内组织、伤道壁5～10mm内组织细菌含量无统计学差异(P>0．05)，各组内随着离伤道距离的增加细菌含量减少(P<0．01)。3组创面组织、伤道壁5mm内组织细菌含量与D组比较差异显著(P<0．01)。结论 高温高湿环境对颅脑火器伤6h内的细菌生长情况无显著影响，颅脑火器伤后6h内细菌繁殖未进入高速增长期，进行清创仍是安全的。

Objective To investigate the growth behavior of characteristics in craniocerebral gunshot wound of cats in a hot and humid environment. Methods Twenty-three cross-bred cats were randomly divided into 4 groups: group A, the gunshot wound control group at normal temperature, in which tissue sampling was performed immediately after the wounding; group B, another gunshot wound control group at normal temperature, in which the samples were taken 6 h after the wounding; group C, the gunshot wound group subjected to a hot and humid environment, in which the tissue samples were obtained 6 h after the wounding; group D, the control group without undergoing the wound. The tissues from the wound tract and the surrounding tissues were sampled for bacterial culture and counting. Results The bacterial counts of the tissues from the wound tract, the tissues within 5 mm and within 5-10 mm from wound tract varied insignificantly between groups A, B and C (P>0.05). In each group, the bacterial counts declined in the tissues as the distance of the sampling sites from the wound tract increased (P<0.01). The bacterial counts of the tissues from the wound tract and within 5 mm from the wound tract in group A, B and C were significantly different from those in group D (P<0.01). Conclusion Hot and humid environment does not significantly affect the bacterial growth in the craniocerebral gunshot wound within the first 6 h, which is a safe period against rapid bacterial growth and suitable for debridement.