银杏外种皮中银杏酸的体外抗肿瘤活性研究

Study on Antitumor Activities of Ginkgolic Acids from Ginkgo Sarcotestas in Vitro

目的 :探讨银杏酸对肿瘤细胞和正常细胞生长的影响。方法 :从银杏外种皮中制备纯度 90 %的银杏酸同系混合物。采用MTT法 ,检测银杏酸对各细胞株生长的抑制作用。结果 :银杏酸浓度为 5 0 μg/ml时 ,对肿瘤细胞生长都有明显的抑制作用 ,其中对肺癌LTEP a 2细胞抑制率达到 5 9 1% ,而对正常细胞无影响 ;高浓度银杏酸对正常细胞和肿瘤细胞生长的抑制作用趋于一致。结论 :银杏酸对体外培养的肿瘤细胞有较强抑制作用 ,浓度低于5 0 μg/ml时对正常细胞生长无影响。

Objective: To study the influence of ginkgolic acids on human tumor cells and normal cells. Methods: Ginkgolic acids (total concentration 90%) was prepared from ginkgo sarcotestas. The inhibitive effect of ginkgolic acids on human tumor cells and normal cells lines was examined by MTT assay. Results: When the concentration was 5.0μg/ml, ginkgolic acids obviously inhibited the growth of tumor cells and didn’t influence the normal cells. Inhibitive rate of ginkgolic acids on LTEP-a-2 was 59.1%. High-concentration ginkgolic acids had inhibitive effect on the growth of tumor cells and normal cells. Conclusion: Ginkgolic acids had a obvious inhibitive effect on tumor cells in vitro. When the concentration was under 5.0 μg/ml, ginkgolic acids didn’t influence the growth of normal cells.