活血补气方对胎鼠宫内发育迟缓的防治效果及其机制

Therapeutic Effect and Its Mechanism of “Huo Xue Bu Qi Fang” on Fetal Rats with Intrauterine Growth Retardation

目的 :探讨活血补气方 (丹参、川芎、当归、黄芩和黄芪 )对胎鼠宫内发育迟缓 (IUGR)的防治效果及其机制 ,为该方剂的临床应用提供实验依据。方法 :被动吸烟法建立孕鼠IUGR妊娠模型。IUGR妊娠鼠被随机分为 4组 (每组 10例 ) ,即中药高、中、低剂量干预组和未干预组 ,每天分别给予活血补气方汤药 3、1、0 3ml/ 10 0g和生理盐水 1ml/ 10 0g。另选 10只正常妊娠鼠作为正常对照组 ,每天给予生理盐水 1ml/ 10 0g。于妊娠第 2 1天处死孕鼠并剖腹取胎 ,测定胎鼠体重、鼻 臀长 ,并计算胎仔重量系数。分别用硝酸还原酶法、放射免疫分析法 (RIA)和酶免疫法(EIA)测定妊娠晚期孕鼠血NO、ET 1含量和尿 8 epi PGF2α含量。结果 :与未干预组比较 ,各干预组的胎鼠体重、鼻 臀长及胎仔重量系数均明显增加 ,差异具有显著性意义 (均为P <0 0 1) ;干预组的孕鼠血NO水平升高 ,血ET 1水平降低 ,NO/ET 1比值升高 ,尿 8 epi PGF2α水平降低 ,差异具有显著性意义 (均为P <0 0 1)。结论 :IUGR妊娠时 ,孕鼠体内脂质过氧化作用增强 ,并存在着NO/ET 1失衡 ,可导致胎鼠IUGR的发生 ;活血补气方具有抗氧化的功能 ,能抑制IUGR妊娠的脂质过氧化作用和调节NO/ET 1平衡 ,对胎鼠IUGR具有较好的防治效果。

Objective:To probe into therapeutic effect and its mechanism of “Huo Xue Bu Qi Fang” (HXBQF) on fetal rats with intrauterine growth retardation (IUGR).Method:The model of pregnant rat with IUGR was established by passive smoking method.Forty pregnant rats with IUGR were randomly divided into intervention group (with high-,middle- and low-dose Chinese traditional medicine) and non-intervention group.In addition,10 normal pregnant rats were taken into control group.Intervention group was adminstered with 16.2,5.4 and 1.62 g/kg HXBQF respectively. Non-intervention group and control group were administered with 10 ml/kg saline.Fetal rats were taken out,and blood and urine samples were collected from pregnant rats on day 21 of the pregnancy.The weight,nose-hip-length and poundera index of these fetal rats were measured.Serum NO,plasma ET-1 and urine 8-epi-PGF 2α level from pregnant rats were determined by nitro-reductase method,radioimmunoassay (RIA) and enzyme immunoassay (EIA) respectively.Result:Compared with fetal rats from non-intervention group,fetal weight,distance between nose-hip, poundera index serum NO level and NO/ET-1 were increased,plasma ET-1 level and urine 8-epi-PGF 2α level were decreased in those from intervention group (P<0.01). Conclusion:There is an enhancement of lipid peroxidation and NO/ET-1 ratio imbalance in pregnant rats with IUGR.HXBQF has good therapeutic effect on IUGR since it can inhibit lipid oxidation and regulate NO/ET-1 balance.