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口蹄疫病毒多基因腺病毒穿梭质粒的构建 被引量：4

Construction of recombinant adenoviral pShuttle-CMV plasmid containing multiple genes of FMDV
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摘要 采用分步扩增连接的方法将口蹄疫病毒结构蛋白基因P1 2A、3C蛋白酶基因和 3D聚合酶基因按正确的读码框依次连接克隆入 pGEM T载体。然后 ,将切取的目的基因亚克隆入腺病毒穿梭质粒 pShuttle CMV。构建成功的 2个重组穿梭质粒经测序鉴定 ,含有完整的目的基因表达盒 ,穿梭质粒可与腺病毒骨架载体进行细菌内同源重组 。 Two recombinant adenoviral pShuttle-CMV plasmid containing FMDV type O capsid , 3C protease and 3D polymerase coding regions were constructed by the methods of multi-step cloning and ligating PCR. The recombinant pShuttle-CMV plasmids were confirmed by PCR,digestion of restricted endonuclease and sequencing.These recombinant pShuttle-CMV can be used to create recombinant adenovirus containing FMDV capsid and no-structural protein genes.
作者 卢曾军 曹轶梅 刘在新 谢庆阁
机构地区 中国农业科学院兰州兽医研究所农业部畜禽病毒学重点开放实验室
出处 《中国兽医科技》 CSCD 北大核心 2004年第2期21-24,共4页 Chinese Journal of Veterinary Science and Technology
基金 国家"973"项目 (G19990 1190 3)
关键词 口蹄疫病毒 腺病毒 穿梭质粒 结构蛋白基因 FMDV adenovirus pShuttle vector
分类号 S852.659.6 [农业科学—基础兽医学]
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参考文献10

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同被引文献39

  • 1刘铀,毕英佐,曹永长,马静云,肖俊芳.新城疫病毒HN基因与T4噬菌体SOC基因在大肠埃希氏菌中的融合表达[J].中国兽医科技,2004,34(8):27-31. 被引量：2
  • 2吴健敏,任兆钧,余兴龙,张念祖,涂长春.猪瘟病毒E2蛋白重组T4噬菌体的构建及免疫学特性[J].中国兽医学报,2004,24(6):521-524. 被引量：5
  • 3田纯见,毕英佐,曹永长,谢青梅.口蹄疫病毒3C蛋白酶的T4噬菌体表面展示[J].中国兽医科学,2006,36(7):519-522. 被引量：2
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引证文献4

二级引证文献15

中国兽医科技
2004年 第2期

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