羊毛皮张和土壤中炭疽杆菌PCR检测方法的建立

Establishment of detection method of Bacillus anthracis in raw wool, skin and soil by PCR

建立了检测炭疽芽孢杆菌染色体特异性基因序列 (Ba813)的PCR技术 ,并用于模拟污染的羊毛、皮张和土壤中炭疽芽孢 (Sterne菌株、PasteurⅡ菌株 )的检测。结果表明 ,该方法可特异、敏感地检出 0 .5 g羊毛中Sterne菌株的 4 5 2个芽孢和PasteurⅡ菌株的 6 88个芽孢 ;0 .5g皮张中Sterne菌株的 4 5 2个芽孢和PasteurⅡ菌株的 10 32个芽孢 ;0 .5 g土壤中Sterne菌株的 4 5 2个芽孢和PasteurⅡ菌株 1376个芽孢。证实PCR可用于羊毛、皮张和土壤等外环境中炭疽杆菌芽孢的检测。

A PCR technique for detection of Bacillus anthracis gene Ba813 had been established in this experiment, and the raw wool, skin and soil contaminated artificially with B.anthracis was detected by the PCR. The results showed that the PCR can detect sensitively and specifically 452 spores in Sterne strain in 0.5 g raw wool, skin and soil sampels respectively, and 688 spores in Pasteur Ⅱ strain in 0.5 g raw wool, 1032 spores in Pasteur Ⅱ strain in 0.5 g skins and 1 376 spores in Pasteur Ⅱstrain in (0.5) g soil respec-(tively). It was confirmed that the PCR can be used as the method for detection of B.anthracis in wool, skin, soil and like in environment.