摘要
以国内主要流行的猪胸膜肺炎放线杆菌 (APP)QH 1、HN 7菌株的基因组DNA为模板 ,通过PCR方法扩增出外膜脂蛋白 (OML)基因片段 ,然后将其克隆至 pMD18 T载体中 ,经酶切和PCR鉴定 ,对阳性克隆进行序列测定。将测序结果分别与标准菌株进行比较 ,QH 1株的核苷酸序列与血清 1、9、11、12型参考株的同源性达 99.1%～ 99.9% ;HN 7株的核苷酸序列与血清 7、3、4、6型参考株的同源性达 97.3%～ 10 0 % ,与其他血清型参考株的同源性较低。
The DNA was abstracted fromActinobacillus pleuropneumoniae (APP) QH-1 strain and HN-7 strain that are predominantly prevalent strains in China. The fragment of the outer membrane lipoprotein (OML) gene of A.pleuropneumoniae was amplified by polymerase chain reaction (PCR) and cloned into the pMD18-T vector. Positive clones of OML gene were identified by restriction analysis and PCR, and then sequenced. The result of sequence analysis for theORF of OML gene indicated that the nucleotide sequence of QH-1 strain shared 99.1%-100% homologywith that of the reference serotype 1,9,11 and 12, but the nucleotide sequence of HN-7 strain shared 97.3%-99.9% homologywith that of the reference serotype 3,4,6 and 7. The nucleotide sequence of QH-1 strain and HN-7 strain exhibited low homologywith that of other reference strains.
出处
《中国兽医科技》
CSCD
北大核心
2004年第2期17-20,共4页
Chinese Journal of Veterinary Science and Technology
