PCR和ELISA检测不同人群TTV的感染状况

The detection of TTV in different human groups by PCR and ELISA

目的 检测湛江地区不同人群TTV的感染情况。方法 应用PCR和ELISA法同时检测正常献血员血清标本 5 12份 ,HBsAg阳性血清标本 60份 ,抗 HCV阳性血清标本 48份。结果 正常献血员、HBsAg阳性、抗 HCV阳性标本用PCR法检测TTVDNA阳性率分别为 10 .94%、13 .3 3 %和 16.67%;ELISA检测抗 TTVIgG ,阳性率分别为 7.42 %、10 .0 0 %和 10 .42 %。两种检测方法结果差异无显著性 (P>0 .0 5 ) ,不同人群TTVDNA和TTVIgG阳性率差异也无显著性 ( P >0 .0 5 )。结论 该地区献血员人群TTVDNA、TTVIgG与HBsAg阳性、抗

Objective To detect the infection state of TTV in different human groups in Zhanjiang district.Methods The 620 blood specimens were detected for TTV DNA and TTV IgG by PCR and ELISA,including 512 specimens from normal blood donors,60 HBsAg positive specimens and 48 anti HCV positive specimens.Results The positive rates of TTV DNA in the three groups were 10.94%,13.33% and 16.67% respectively.At the same time,the positive rates of TTV IgG were 7.42%,10.00% and 10.42% respectively.There was no significant difference in the test results between the two methods (P>0.05).There were no significant differences in TTV DNA and TTV IgG among the three groups (P>0.05).Conclusion The TTV DNA and TTV IgG in normal blood donors are not correlated to HBsAg positivity and anti HCV positivity.