咽部链球菌感染与银屑病皮损角蛋白14表达关系的研究

Detection of keratin 14 in lesions of psoriasis associated with pharyngeal streptococcal infection

目的 为研究银屑病皮损角蛋白 14 (K 14 )的表达情况 ,探讨与含M6蛋白链球菌感染的关系。方法 对寻常型银屑病患者进行咽部细菌培养、鉴定 ,链球菌阳性者分为含M6蛋白组及不含M6蛋白组。两组皮损和正常皮肤组织链霉亲合素 -过氧化酶 (S -P)免疫组化染色后 ,用光学显微镜描述并图像分析定量统计。结果 银屑病表皮全层表达K14 ,正常皮肤主要在基底层浓集表达 ,咽部培养出含M6蛋白链球菌的银尿病患者比不含M 6蛋白链球菌者有更密、更深染的K14阳性颗粒 (P <0 0 5)。结论 银屑病表皮过度表达K14 ,咽部感染含M 6蛋白链球菌的银屑病患者比感染不含M

ObjectiveTo study keratin 14(K14)expression in psoriatic les ions and investigat e the relationship between K14 expression and streptococcal infection.Me thodBac teria were isolated from throats of psoriatic patients.Patients with streptococc i were divided into two groups by culture results(group with streptococci contai ning M6 protein and group with streptococci non-containing M6 protein).Immunohis tochemical streptavidin-peroxidase technique was used to stain psoriatic lesions and normal control skins.The slides were observed descriptively through microsc opy and quantified with pathological image assay system.Results The results show ed that K14 express increasingly in the whole psoriatic epidermis,but in normal c ontrols,it expressed only in basal layer.Patients with streptococci containingM6 protein had denser and darker positive particles than those with streptococci non-containing M6 protein(P<0 05)。 ConclusionIt indicate that K14 express overly in psoriatic epidermis ,especially in patients infecting streptococcus containing M6 protein.