血管内皮生长因子反义寡核苷酸治疗肺癌的实验研究

Effect of VEGF antisense oligonucleotides on growth of Lewis lung cancer

目的 探讨血管内皮生长因子 (VEGF)反义寡核苷酸对C57BL 6小鼠肺癌的抑制作用。方法 制作C57BL 6小鼠皮下肺癌模型 30只 ,分为VEGF反义寡核苷酸 (ASODN)治疗组、VEGF正义寡核苷酸 (SODN)治疗组及对照组。接种Lewis肺癌细胞后 2 4h内 ,用ASODN及SODN皮下注射治疗 ,对照组只注射生理盐水 ,观察小鼠肿瘤的生长情况以及组织形态学改变。标本常规石蜡切片 ,HE染色。结果 对照组、VEGF反义寡核苷酸治疗组、VEGF正义寡核苷酸治疗组平均瘤重分别为 (7.83± 0 .78)g、(4 .4 9± 0 .4 3)g和 (7.73± 0 .6 9)g。VEGF反义寡核苷酸治疗组、VEGF正义寡核苷酸治疗组抑瘤率分别为 4 2 .7%、5 .9%。结论 原位注射VEGF反义寡核苷酸能抑制小鼠肺癌生长。

Objective To study the inhibitory effect of VEGF antisense oligonucleotides on growth of Lewis lung cancer in C 57 BL/6 mice.Methods The Lewis lung cancer cells were cultured and implanted subcutaneously into 30 C 57 BL/6 mice,and subsequently the mice were divided into three groups:VEGF ASODN group,VEGF SODN group and the control group.Different treatments were given respectively 24 hours after cell inoculation.The weight and volume of subcutaneous tumors were measured,and the morphological changes of tumor cells were observed under microscope.Results The mean tumor weight of the VEGF ASODN group was ( 4.49 ±0.43)g,that of the VEGF SODN group was (7.73±0.69)g,and that weight of the control group was (7.83±0.78)g.The inhibition rates of tumor growth of the VEGF ASODN group and VEGF SODN group were 42.7% and 5.9%, respectively.Conclusion It is showed that the Lewis lung cancer can be inhibited by the VEGF antisense oligonucleotides inoculated into the center of the tumor in the C 57 BL/6 mice.