抗Ia单克隆抗体对异基因小鼠皮肤移植物的影响

Effect of anti-Ia monoclonal antibody on allogeneic skin grafts in mice

目的 探讨抗Ia抗体对移植皮片存活期的影响。方法 用A .TL鼠 (H 2 k)皮肤及淋巴细胞免疫A .TH鼠(H 2 S) ,取脾细胞与骨髓瘤细胞 (P3U1 )融合后 ,经HAT培养基选择性培养 ,得到杂交瘤细胞 ,用补体依赖的细胞毒试验检测上清中的抗体与人B细胞反应的活性 ,阳性孔细胞以有限稀释法进行克隆化 ,得到抗Ia单克隆抗体 ,预先将抗Ia抗体注入异基因皮肤移植的受体小鼠腹腔后 ,观察移植皮片存活期。结果 实验组皮肤移植物存活期与对照组相比明显延长 (P <0 .0 1 )。

Objective To evaluate the function of anti Ia monoclonal antibody on the survival times of allogeneic skin grafts in mice.Methods A.TH(H 2 S) mouse was immunized with skin and lymphocytes of A.TL(H 2 k) mouse,the spleen cells of immunized mouse were fused with the cells of Balb/c myeloma(P 3U 1) in the presence of PEG,the hybrid cells were selected by culturing in the medium supplemented with HAT.After identification,the cells from the wells positive for anti human B cell activity were colonized by limiting dilution.The monoclonal antibodies(anti Ia McAb) were obtained in the form ascites fluid from Balb/c mice injected with the hybridoma cells.The mice of test group get anti Ia McAb injection before allogeneic skin transplantation.The survival time of transplanted skin grafts was observed.Results The survival times of the allogeneic skin grafts of the mice were longer than those of the controls( P <0.01).Conclusion Anti Ia antibody can prolong the survival times of the allogeneic grafts.