β-内啡肽在窒息新生儿细胞免疫功能变化中的作用

EFFECT OF β-ENDORPHIN LEVEL ON CELLULAR IMMUNOLOGIC FUNCTION OF ASPHYXIA NEWBORNS

目的 观察窒息新生儿细胞免疫功能 (T细胞亚群 )及 β -内啡肽 (β -EP)的动态变化 ,并探讨 β -EP是否参与了其中的免疫调节机制。方法 分成窒息 (观察 )组 (41例 )和正常 (对照 )组 (2 0例 ) ,均于生后第一和第七天两次采血 ,分别检测 β -EP、CD3、CD4及CD8等各项指标。结果 与对照组相比 ,观察组新生儿细胞免疫功能明显抑制 ,且与窒息程度成正比 ,而 β -EP则显著升高 :观察组为 (2 0 1 .45± 82 .97)ng/L ,轻度窒息组为 (335 .2 2± 75 .82 )ng/L ,重度窒息组为 (882 .45± 1 1 2 .37)ng/L(P均 <0 .0 1 ) ;两组第二次检测显示 β-EP、CD3、CD4/CD8等项差异有显著性 (P <0 .0 5) ;观察组 β-EP与各免疫指标两次检测差值的相关分析示 :除CD8外 ,均有显著相关性 (P <0 .0 5) ,相关系数 (r)在 0 .457～ 0 .72 8之间。结论 窒息新生儿细胞免疫功能明显抑制 ,且与窒息程度成正比 ,β-EP参与了其细胞免疫的抑制性调节。

Objective To observe the dynamic changes of cellular immunologic function (T-cell subpopulation) and β-endorphin(β-EP) level of asphyxia newborns,and to explore β-EP's regulation mechanism of cellular immune. Methods All cases were divided into two groups: asphyxia group (45 cases) and normal group(20 cases). Two blood samples were collected at the first day and the seventh day after birth,β-EP, were detected. Results Compared with normal group,cellular immunologic function of asphyxia group were inhibited obviously and the degree was positive to asphyxial degree.Level of β-EP increased significantly,(201.45±82.97)pg/ml in normal group,(335.22±75.82)pg/ml in mild asphyxia group,(882.45±112.37)pg/ml in severe asphyxia group(P<0.01).There were significant difference in level of β-EP,CD3,CD4,CD8 among groups(P<0.05).To asphyxia group,there were significant correlation between level of β-EP and immunologic indexes except CD8,correlation coefficient (r)was 0.457～0.728. Conclusion Cellular immunologic function of asphyxia newborns are inhibited obviously and inhibited degree is positive to asphyxial degree,β-EP play a role in inhibited regulation of the cellular immunologic function.