PTEN基因蛋白在恶性黑素瘤中的表达及意义

Expression of tumor suppressor gene protein PTEN in malignant melanoma

目的 探讨PTEN基因蛋白在恶性黑素瘤中的表达及其意义。方法 采用免疫组化EnVision检测PTEN基因蛋白在 5 1例恶性黑素瘤以及 30例皮内痣组织中的表达情况。结果 恶性黑素瘤组织中PTEN表达强度低 ,总体阳性率 5 2 .9%(2 7/5 1 ) ,其中Ⅰ、Ⅱ、Ⅲ各期阳性率分别为 5 9.4 % (1 7/32 )、38.5 % (5 /1 3)、33.3% (2 /6 ) ;而皮内痣中PTEN均呈强阳性 ,表达阳性率为 1 0 0 % (30 /30 ) ,二者差异具有显著性 (P <0 .0 1 )。结论 恶性黑素瘤的发生可能与PTEN基因蛋白的低表达有关 ,PTEN基因蛋白表达缺失或低下者更易发生远处转移 。

Objective To investigate the expression and significance of tumor suppressor gene protein PTEN in malignant melanoma.Methods Immuohistochemistry technique (EnVision method) were performed to detect the expression of PTEN protein in melanomas and intracutaneous nevi.Results High expression of PTEN protein was detected in all 30 cases of intracutaneous nevi. The general positive rate in malignant melanoma was 52.9 %(27/51), and the positive rates at Ⅰ、Ⅱ and Ⅲ stage were 59.4 %(17/32)、 38.5 %(5/13)、 33.3 %(2/6), respectively. The statistical diference between these two groups was prominent (P< 0.01 ).Conclusion Abnormal expression of PTEN is associated with the development and progression of malignant melanoma, which suggests PTEN is one of new markers to evaluate the patient′s prognosis.