无溶剂体系中酶催化亚油酸、甘油生产1,3-甘油二酯工艺的研究

Enzymatic production of 1,3-diacylglycerol from linoleic acid and glycerol in a solvent free system

以亚油酸、甘油为原料,用专一性脂肪酶在无溶剂系统中快速合成了1,3 dilinolein,实验发现真空脱水可有效增加亚油酸的转化率,使反应平衡向合成方向移动。不同反应温度、底物摩尔比条件下的酯化进程曲线对比研究表明,温度、底物摩尔比不仅影响酯化反应的初速度、转化率,还与产物组成密切相关。60℃、亚油酸与甘油摩尔比为2∶1,0.01MPa真空脱水,反应8h亚油酸酯化率可达90.2%。产品经分子蒸馏纯化后1,3 DAG可达84.25%

Enzymatic synthesis of 1,3-dilinolein was studied by using linoleic acid and glycerol as substrate for Lipozyme RM IM in a solvent free system.The time course of linoleic acid glyceride was compared under different temperature and molar ratio of substrate.The results show that conversion rate of linoleic acid can be increased and the reaction equilibrium shiftes to 1,3-dilinolein synthesis direction with water removal in vaccum.Temperature and molar ratio affect the initial reaction velocity,conversion rate,as well as the products composition.A conversion of 90.2% can be obtained when reacting at 60℃ for 8 hours,molar ratio of linoleic acid and glycerol 2∶1,and removal of water in vacuum.After purification by molecular distillation,content of 1,3-DAG in the product can reach 84.25%.