急性低氧条件下大鼠循环血中内皮细胞及肺小动脉内皮细胞的改变

THE CHANGE OF CIRCULATING ENDOTHELIAL CELL IN BLOOD OF RATS AND PULMONARY SMALL ARTERY VASCULAR ENDOTHELIAL CELL UNDER ACUTE HYPOXIA

目的 :探讨急性低氧对血管内皮细胞 (VEC)和循环血液中内皮细胞 (CEC)的影响。方法 :用光镜观察急性低氧情况下血中CEC的形态并记数 ,用组织化学染色和显微分光光度计对血中年轻型CEC及肺小动脉VEC的乳酸脱氢酶 (LDH)活性进行定量分析。结果 :与低氧前相比 ,低氧后各组VEC的LDH活性均下降 ,且随低氧时间延长 ,LDH活性下降越明显。而低氧前后各组年轻型CEC的LDH活性是一致的 ,且明显低于各组VEC的LDH活性。低氧前CEC多为衰老型 ,低氧后CEC数量增多且多为年轻型 ,随低氧时间延长年轻型CEC增多越明显。结论 :CEC的形态和数量的变化可以反映血管的损伤情况 ;VEC的LDH活性可以反映VEC向CEC转化的进程 ;年轻型CEC的LDH活性可以反映VEC脱落时的损伤程度。

Aim: To investigate changes of VEC and CEC under acute hypoxia. Methods: Observe CEC in blood under acute hypoxia morphologically and count the number of CEC by opticalmicroscope,measure LDH activity of young CEC and VEC by histochemical staining image analysis. Results: LDH activities of VEC in hypoxic groups are lower than that in the group before hypoxia and decrease progressively with hypoxia time. LDH activities of young CEC in groups after hypoxia and before hypoxia are the same and are apparently lower than that of VEC. Before hypoxia most of CEC are aging,the number of CEC from hypoxic groups is greater than that before hypoxia and increases progressively with hypoxia time and most of CEC from hypoxic groups are young. Conclusion: The morphology and number of CEC may reflect the extent to which the vascular is injured. LDH activity of VEC may reflect the transformation from VEC into CEC. LDH activity of young CEC may reflect the extent to which the VEC is injured when falling from vascular wall.