牛磺酸对失血性休克再灌注肺损伤的防护作用及其机制探讨

RELATIONSHIP BETWEEN LUNG INJURY INDUCED BY HEMORRHAGIC SHOCK/REPERFUSION AND NITRIC OXIDE AND BENEFICIAL EFFECT OF TAURINE

目的 :探讨失血性休克再灌注肺损伤与一氧化氮的关系及牛磺酸对其的影响。方法 :健康家兔 2 4只随机分为三组 :对照组、单纯休克组、牛磺酸治疗组。采用失血性休克再灌注后肺损伤模型。测定肺组织及血浆中一氧化氮合酶 (NOS)活性、一氧化氮代谢产物 (NO2 -/NO3 -)含量、超氧化物歧化酶 (SOD)活性、丙二醛 (MDA)含量、肺湿重/肺干重、肺水含量、肺通透指数 (LPI)、肺泡灌洗液 (PALF)中蛋白含量等指标的变化 ,并常规留取肺标本进行病理形态观察。结果 :①再灌注 3h时肺组织及血浆中SOD活性显著下降 ,而上述其它指标均显著升高 ,与对照组相比差异有显著性 (均P <0 .0 1)。②血浆、肺组织中NO2 -/NO3 -含量与MDA含量均呈正相关 ,且肺组织中NO2 -/NO3 -含量和肺损伤指标呈显著正相关。③牛磺酸 (40mg·kg-1,iv)可减轻上述指标的变化。结论 :NO在休克再灌注肺损伤中起重要作用 ,牛磺酸可减少NO的生成、增强自由基的清除从而使肺组织损伤减轻。

Aim: To approach the relationship between lung injury induced by shock/reperfusion and nitric oxide as well as the beneficial effect of taurine. Methods: Twenty four rabbits were divided randomly into 3 groups(n=8): control group,shock group,taurine group. The model of lung injury induced by shock/reperfusion was used. The activities of nitric oxide synthase(NOS),superoxide dismutase(SOD),the contents of malondialdehyde(MDA),nitric oxide products( NO 2 -/NO 3 -) in plasma and lung homogenate,lung wet/dry weight,lung water content,lung permeability index,and protein content in the pulmonary alveolar lavage fluid were measured. Meanwhile,pathologic samples treated routinely.Results:①At 3 hours after reperfusion,the activities of SOD in plasma and lung homogenate decreased markedly,but the other indexes above mentioned were increased significantly compared with the control group(P<0.01). ② A close correlation was shown between MDA content and NO 2 -/NO 3 - content in plasma and lung.Furthermore,the content of NO 2 -/NO 3 - in lung homogenate showed strong positive correlation with the lung injury parameters.③Taurine(40 mg·kg -1 iv) could attenuate all the changes above mentioned at the same time points of reperfusion.Conclusion: NO may play an important role in lung injury induced by shock/reperfusion Taurine can ameliorate the lung injury,mechanism of which may be related to decreasing the generation of NO and anti-lipoperoxidation.