排卵正常妇女子宫内膜种植窗期基质金属蛋白酶-9及其抑制物-1的表达特点及作用

Expression of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 protein in the implantation window phase of endometrium in ovulation normal women

目的 :探讨基质金属蛋白酶 - 9(MMP - 9)和组织金属蛋白酶抑制物 - 1(TIMP - 1)在排卵正常妇女子宫内膜种植窗期的表达特点 ,及其在胚泡植入中的作用。方法 :选择排卵正常妇女的子宫内膜 ,用扫描电镜 (SEM )确定分泌中期子宫内膜种植窗开放 ;用免疫组化法检测 15例种植窗期子宫内膜组织中MMP - 9/TIMP - 1蛋白的表达 ,并与 15例增生期、13例分泌早期、14例分泌晚期内膜的表达相比较。结果 :MMP - 9/TIMP - 1在各期子宫内膜中均呈阳性表达 ,腺上皮细胞、腔上皮细胞和基质细胞胞浆内均可见棕黄色颗粒沉着。从增生期到分泌早期至种植窗期染色逐渐增强 ,以种植窗期最明显 (P <0 .0 5 ) ,分泌晚期略低于种植窗期 ,但明显高于分泌早期和增生期 (P <0 .0 5 )。结论 :排卵正常妇女种植窗期子宫内膜高表达MMP - 9/TIMP - 1蛋白 ,可能参与了胚泡的着床 ,调节胚胎的植入过程。

Objective:To study the expression of matrix metalloproteinase-9 (MMP-9)/tissue inhibitor of metalloproteinase-1(TIMP-1) protein in the implantation window endometrium phase in ovulation normal women and the role of MMP-9/TIMP-1 on embryo implantation.Methods:SEM was used to determine “implantation window” of the mid-secretive phase endometrium in ovulation-normal women.Immunohistochemical analysis was applied to measure the expression of MMP-9/TIMP-1 protein in the implantation window in 15 cases,and MMP-9/TIMP-1 expression in endometrium during different phases were compared.Results:MMP-9/TIMP-1 expression in endometrium was observed in luminal,glandular epithelium and stromal cells in different phases.The expression was lower in proliferative phase,and got to the summit in the implantation window and late secretive phase and it expressed significantly higher in the implantation window phase(P<0.05).There was no difference between the implantation window and late secretive phase.Conclusion:Expression of MMP-9/TIMP-1 in the implantation window phase of endometrium is specific in ovulation normal women and may play an important role in regulating the depth of trophoblast invasion during embryo implantation.