阿霉素诱导兔眼后囊晶状体上皮细胞凋亡及其凋亡基因的表达

Apoptosis of posterior capsular lens epithelial cell induced by adriamycin and the expression of apoptosis-related gene in rabbit

目的 :探讨阿霉素对兔晶状体摘除术后诱导后囊晶状体上皮细胞凋亡并观察凋亡相关基因 (P5 3、Bcl- 2 )的表达。方法 :在 12只兔眼晶状体囊外摘除术中分别注入 0 .2ml阿霉素 (0 .4mg/L) ,在术后 3周、8周分别行TUNEL法和免疫组化SABC法染色 ,计算P5 3、Bcl- 2基因表达阳性百分率。结果 :术后 3、8周TUNEL法标记的阳性凋亡细胞比对照组明显增多 (P<0 .0 5 )。P5 3蛋白表达阳性率在 3、8周比对照组显著增多 (P <0 .0 1)。Bcl- 2蛋白表达阳性率在 3周时显著减少 (P <0 .0 1) ,在 8周时仍比对照组减少 (P <0 .0 5 )。结论 :阿霉素诱导兔后囊晶状体上皮细胞凋亡 ,用药组P5 3基因表达而Bcl- 2基因低表达 ,提示P5 3、Bcl- 2基因可能参与晶状体上皮细胞凋亡的调控。

Objective:To study the effect of adriamycin(ADR) as therapeutic agent inducing rabbit posterior capsular lens epithelial cell apoptosis and to assess the relationships between P53,Bcl-2 after lens extracapsular extraction.Methods:Twelve rabbit eyes were injected with 0.2mg ADR(0.4mg·L -1 ) during lens extracapsular extraction performed respectively in the third week and the eighth week after the operation.With TdT-mediated dUTP nick end labeling(TUNEL)and immunohistochemical(SABC) staining.The protein expression cell rate of P53 and Bcl-2 were detected.Results:Positive pericyte muclei labeled by TUNEL increased more significantly than those in the control guoup from the third week to the eighth week after the operation.P53 protein expression was significantly higher(P<0.01) and Bcl-2 protein expression was significantly lower(P<0.05) than those in the control group in the third week and the eighth week after the opreration.Conclusion:ADR-induced rabit posterior capsular lens epithelial cell apoptosis,P53 protein expression is higher and Bcl-2 protein espression is lower in ADR group eyes.P53 and Bcl-2 may play an important role in modulatong the lens epithelial cell apoptosis.