尿胰蛋白酶抑制剂对鼠溃疡性结肠炎结肠组织中蛋白酶活性的抑制作用

Inhibitory effect of urinary trypsin inhibitor on protease activity in the colonic tissues of ulcerative colitis rats

目的 :确定尿胰蛋白酶抑制剂 (UTI)对鼠溃疡性结肠炎 (UC)结肠组织中蛋白酶的作用。方法 :用 18%的DNBS乙醇溶液 0 .2 5ml灌肠 ,在Wistar大鼠上建立UC模型 ;经尾静脉注射UTI ;用明胶酶谱法测定病变结肠组织中的蛋白酶活性。结果 :经UTI处理后 ,鼠UC结肠组织中蛋白酶活性仅为对照组的 1/3。结论 :UTI能显著降低鼠UC结肠组织中蛋白酶活性。

Objective:To determine the effect of urinary trypsin inhibitor(UTI)on protease activity in the colonic tissues of ulcerative colitis(UC) rats.Methods:The experimental UC was induced in Wistar rats by enema with 0.25ml of 18% 2,4-dinitrobenzenesulfonic acid (DNBS) dissolved in ethanol.UTI was injected into the rats through the caudal vein.Protease activity present in colonic tissues was determined on gelatin zymograms.Results:The protease activity which presented in colonic tissues of the group treated with UTI was only 1/3 that of the control.Conclusion:The protease activity decreased significantly after we treated the rats with UTI.